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[Determination of rhein in plasma of rat by HPLC/MS]
Chenchen Zhu1, Zhihua Zheng, Zhiliang Chen
1New Drug R&D Center, Guangzhou University of Traditional Chinese Medicine, Guangzhou 510405.
Zhong Yao Cai = Zhongyaocai = Journal of Chinese Medicinal Materials
|November 28, 2002
Abstract:
A HPLC/MS method has been established for the determination of rhein in rat plasma, using an Alltima C18 column (4.6 x 250 mm, 5 microns) and a mixture of ACN-water (60:40) as the mobile phase, at the flow rate of 0.8 ml/min with the column temperature at 25 degrees C. The results showed that the level of rhein distributed in plasma reached the max value 0.5 h after the oral administration, and maintained a relatively high value from 0.25 h to 1.5 h. The method possessed high selectivity and sensitivity.