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Electrophoretic separations with polyether ether ketone capillaries and capacitively coupled contactless conductivity
Jatisai Tanyanyiwa1, Sandro Leuthardt, Peter C Hauser
1University of Basel, Department of Chemistry, Spitalstrasse 51, 4004 Basel, Switzerland.
Journal of Chromatography. A
|December 3, 2002
Summary
Polyether ether ketone (PEEK) capillaries are compatible with contactless conductivity detection (C4D). This enables efficient separation and analysis of both inorganic and organic ions in a single run, offering a versatile analytical method.
Area of Science:
- Analytical Chemistry
- Separation Science
- Materials Science
Background:
- Capacitively-coupled contactless-conductivity detection (C4D) is a sensitive detection method for ion analysis.
- Polymer-based capillaries offer potential advantages over fused-silica in certain applications.
- Compatibility of novel materials with established analytical techniques is crucial for method development.
Purpose of the Study:
- To evaluate the compatibility of Polyether ether ketone (PEEK) capillaries with C4D.
- To determine the detection limits achievable with PEEK capillaries using high excitation voltage C4D (HV-C4D).
- To assess the separation capabilities of PEEK capillaries for inorganic and organic anions.
Main Methods:
- Utilized Polyether ether ketone (PEEK) capillaries for capillary electrophoresis (CE).
- Employed capacitively-coupled contactless-conductivity detection (C4D), including high excitation voltage C4D (HV-C4D).
- Investigated the electroosmotic flow (EOF) characteristics of the PEEK capillary.
Main Results:
- PEEK capillaries demonstrated excellent compatibility with C4D.
- Achieved detection limits in the order of 10(-7) M for inorganic cations and anions using HV-C4D.
- Observed a low electroosmotic flow (2.6 x 10(-4) cm2 V(-1) s(-1)) enabling separation of inorganic and slower organic anions without modifiers.
- Successfully separated both fast and slow organic anions alongside inorganic anions.
Conclusions:
- PEEK capillaries are suitable for C4D, offering a robust alternative to traditional materials.
- The low EOF of PEEK capillaries facilitates the simultaneous separation of diverse anionic species.
- The method allows for flexible sample analysis, including the combined analysis of cations and anions in a single experimental setup or sequential runs.