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Updated: Jul 27, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

Published on: August 5, 2008

Comparative genomic hybridization: practical guidelines.

Judith W M Jeuken¹, Sandra H E Sprenger, Pieter Wesseling

¹Department of Neurology, University Medical Center Nijmegen, The Netherlands. j.jeuken@czzorlnm.azn.nl

Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B

|December 3, 2002

Summary

This paper details comparative genomic hybridization (CGH), a method for detecting genome-wide copy number changes. It provides a comprehensive protocol and troubleshooting guide for researchers performing CGH analysis in human neoplasms.

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Last Updated: Jul 27, 2026

Technical Demonstration of Whole Genome Array Comparative Genomic Hybridization

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Published on: August 5, 2008

Array Comparative Genomic Hybridization (Array CGH) for Detection of Genomic Copy Number Variants

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Published on: February 21, 2015

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants

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Published on: November 8, 2017

Area of Science:

Genomics
Molecular Biology
Cancer Research

Background:

Comparative genomic hybridization (CGH) is crucial for identifying copy number variations (CNVs) across genomes.
Numerous CGH studies have documented chromosomal imbalances in human neoplasms, yet detailed protocols are rarely published.

Framework:

This review presents a comprehensive overview of CGH techniques, including common pitfalls and challenges.
It details a specific CGH protocol, discussing optimization experiments and critical steps for reliable results.

Implementation:

The paper offers a fully described CGH protocol, serving as a practical guideline for new users.
It highlights optimization strategies and points requiring special attention during CGH experiments.

Implications:

This work aims to guide researchers in establishing sensitive and reliable CGH experiments.
It serves as a valuable troubleshooting resource for those encountering difficulties with CGH analysis.