Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Automated postprocessing of electrospray LC/MS data for profiling protein expression in bacteria.

Tracie L Williams1, Peter Leopold, Steven Musser

  • 1Instrumentation and Biophysics Branch, Center for Food Safety and Applied Nutrition, US Food and Drug Administration, College Park, Maryland 20740, USA.

Analytical Chemistry
|December 5, 2002
PubMed
Summary

This study introduces an automated method for bacterial protein analysis using electrospray LC/MS and Retana software. The approach enhances protein identification and quantification, enabling comparative proteomic studies across bacterial strains.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and Characterization of Candidate Vaccine Viruses against High Pathogenicity Avian Influenza A(H5) Viruses for Rapid Pandemic Response.

The Journal of infectious diseases·2026
Same author

Optimization of Extraction Methods for the Quantification of Proteins in Mammalian Tissues.

Analytical chemistry·2025
Same author

Anti-neuraminidase and anti-hemagglutinin stalk responses to different influenza a(H7N9) vaccine regimens.

Vaccine·2025
Same author

State of the science on assessing developmental neurotoxicity using new approach methods.

ALTEX·2024
Same author

Trust your gut: Establishing confidence in gastrointestinal models - An overview of the state of the science and contexts of use.

ALTEX·2024
Same author

An antibody-free evaluation of an mRNA COVID-19 vaccine.

Biologicals : journal of the International Association of Biological Standardization·2023

Area of Science:

  • Proteomics
  • Bacterial Cell Analysis
  • Mass Spectrometry

Background:

  • Automated protein analysis is crucial for high-throughput bacterial studies.
  • Existing methods for protein profiling can be labor-intensive and lack resolution.
  • Standardized and efficient protein extraction and analysis protocols are needed.

Purpose of the Study:

  • To develop and validate an integrated, automated approach for protein analysis of bacterial cell extracts.
  • To improve the efficiency and accuracy of protein identification and quantification.
  • To enable comparative proteomic analysis across different bacterial strains.

Main Methods:

  • Utilized electrospray liquid chromatography coupled with mass spectrometry (LC/MS) for protein profiling.

Related Experiment Videos

  • Developed and applied the Retana software for automated data analysis, including summing, centroiding, and deconvolution of protein spectra.
  • Compared the developed method against "whole cell" Matrix-Assisted Laser Desorption/Ionization (MALDI) analysis.

    • Main Results:

    • The integrated LC/MS and Retana software approach generated concise protein spectra with retention time and relative abundance data.
    • Demonstrated improved mass resolution and mass accuracy compared to "whole cell" MALDI analysis.
    • Observed a greater number of identified proteins using the new method, enhancing proteome coverage.

    Conclusions:

    • The automated electrospray LC/MS and Retana software provide an efficient and accurate method for bacterial protein analysis.
    • This approach significantly improves upon traditional methods like MALDI, offering better resolution and sensitivity.
    • The ability to normalize protein abundance facilitates comparative proteomic studies essential for understanding bacterial strain variations.