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Related Experiment Videos

Xenopus hoxc8 during early development.

Chemyong Ko1, Hae Moon Chung

  • 1Department of Clinical Sciences, University of Kentucky, 900 South Limestone, Lexington 40536, USA. cko2@uky.edu

Biochemical and Biophysical Research Communications
|December 14, 2002
PubMed
Summary
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Xenopus hoxc8 (Xhoxc8) gene is crucial for abdominal structure formation during embryonic development. Its expression is essential, as blocking it leads to severe malformations and embryonic death.

Area of Science:

  • Developmental biology
  • Molecular genetics
  • Xenopus laevis research

Background:

  • Vertebrate hoxc8 genes are implicated in vertebral development.
  • Understanding hoxc8 function in non-mammalian vertebrates is key.

Purpose of the Study:

  • Isolate and characterize Xenopus hoxc8 (Xhoxc8).
  • Investigate the spatio-temporal expression pattern of Xhoxc8.
  • Determine the functional role of Xhoxc8 in embryonic development.

Main Methods:

  • Isolation of Xhoxc8 gene.
  • Analysis of Xhoxc8 mRNA expression using whole-mount in situ hybridization.
  • Retinoic acid treatment experiments.
  • Antisense RNA injection in early Xenopus embryos.

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Main Results:

  • Xhoxc8 shares 94% amino acid identity with mouse hoxc8.
  • Xhoxc8 expression is dynamic, initially broad then restricted to trunk/abdominal regions.
  • Retinoic acid did not alter Xhoxc8 expression patterns.
  • Antisense-mediated knockdown of Xhoxc8 caused severe abdominal malformations and lethality.

Conclusions:

  • Xhoxc8 plays a critical role in the formation of abdominal structures in Xenopus.
  • The expression pattern of Xhoxc8 is essential for normal abdominal development.