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Gene expression profiling using advanced mass spectrometric approaches.

Ljiljana Pasa-Tolić1, Mary S Lipton, Christophe D Masselon

  • 1Environmental Molecular Sciences Laboratory, MSIN: K8-98, Pacific Northwest National Laboratory, PO Box 999, Richland, Washington 99352, USA. ljiljana.pasatolic@pnl.gov

Journal of Mass Spectrometry : JMS
|December 19, 2002
PubMed
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This study introduces a novel comparative proteomics approach. It combines advanced separation techniques with Fourier transform ion cyclotron resonance mass spectrometry for comprehensive, quantitative proteome analysis in systems biology.

Area of Science:

  • Systems biology
  • Proteomics
  • Biochemistry

Background:

  • Systems biology integrates computational and high-throughput experimental approaches.
  • Understanding biological systems requires global analysis of genomes and proteomes.
  • Identifying changes in protein concentration, state, and location is crucial.

Purpose of the Study:

  • To develop a comprehensive and quantitative proteome measurement approach.
  • To identify proteins with altered concentration, post-translational state, or location.
  • To enhance molecular-level understanding of biological systems.

Main Methods:

  • Utilizing high-performance nano-scale separations.
  • Employing Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR-MS).

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  • Combining separation with high-mass measurement accuracy, resolving power, and sensitivity.

    • Main Results:

    • Achieved broad dynamic range in proteome measurements.
    • Enabled comprehensive proteome profiling.
    • Provided quantitative proteome data.

    Conclusions:

    • The described approach offers a powerful tool for comparative proteomics.
    • This method facilitates detailed molecular understanding in systems biology.
    • It supports the identification of disease-specific proteomic alterations.