[Recent advances of heterologous gene expression in E. coli]

In recent years, along with the rapid development of genetic engineering technologies, a vast amount of valuable proteins have been expressed in E. coli with high productivity. The problems concerning the productivity and purity of the heterologous proteins are no longer the major ones, as many expression system and protein purification schemes have been developed and perfected. More attention is being payed to the activity, specific activity, correct folding and intactness of the heterologous proteins. Perhaps it is the solutions to such problems that will finally lead to the maturity of the application of recombinant proteins.