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[cDNA array in the establishment of a gene expression profile associated with differentiation inducing the glioma
Lijun Sun1, Qiang Huang, Aidong Wang
1Department of Neurosurgery, Second Hospital, Suzhou University, Suzhou 215004, China.
Objective:
Establishment of a gene expression profile associated with differentiation inducing the glioma cells was made possible.
Method:
The expression level of 18 000 genes in glioma cells was evaluated before and after induction with sodium phenyl-butyrate for 2 hours or 6 days by cDNA array technique, with the results proved by multi-dot blot.
Results:
Ninety-eight gene expressions in the glioma cells were changed after the induction, with some genes in transcription and translation systems down-regulated, some oncogenes down-regulated, and some differentiation or apoptosis genes up-regulated. Eighteen unknown EST fragments were changed also.
Conclusion:
A gene expression profile associated with differentiation-inducing the glioma cells including 98 genes has been established.
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