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[Hepatitis B virus antigen-induced specific TCR V beta gene subfamily amplifications and their diversity]

S Huang1, M Cao, L Luo

  • 1Department of Molecular Biology, Guangdong Pharmacy College, Guangzhou 510224.

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology
|January 16, 2003
PubMed

The human immune system confronts various antigens, then the recognitive structures of T lymphocytes must be diverse. In this study, we analyzed the TCR V beta 1-20 gene repertoire by reverse transcription-polymerase chain reaction (RT-PCR) in peripheral blood lymphocytes (PBLs) from individuals vaccinated with recombinant hepatitis B vaccine and PBLs from healthy donors stimulated by supernatants of the HepG2 2215 cell line transfected by HBV-DNA. The specific amplifications of V beta 6, 14, V beta 6, 15 were observed in PBLs after vaccination and stimulation by HepG2 2215 cell line respectively. The results suggest that the V beta segments may be the specific recognitive units for these antigens and involved in restriction and cytotoxicity.

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