Related Experiment Videos
[Detection of GBV-C/HGV infection in patients with liver diseases]
1Dept. of Infectious Diseases of Capital University of Medical Sciences, Beijing 100054.
Insights
The Hepatitis G virus (HGV), also known as GBV-C, was found in 9.5% of liver disease patients in Beijing. HGV infection prevalence was higher in patients with a history of surgery or transfusion.
Area of Science:
- Hepatology
- Virology
- Infectious Diseases
Context:
- Hepatitis G virus (HGV), also known as GBV-C, is a significant cause of viral hepatitis.
- Understanding the prevalence and clinical impact of HGV is crucial for patient management and public health.
- Previous studies on HGV prevalence in liver disease patients in China are limited.
Purpose:
- To determine the prevalence of GBV-C/HGV infection in patients with various liver diseases.
- To investigate the clinical features associated with GBV-C/HGV infection.
- To analyze the genetic characteristics of HGV isolates in the studied population.
Summary:
- A study of 169 liver disease patients in Beijing found a 9.5% prevalence of GBV-C/HGV RNA.
- HGV RNA positivity was significantly higher (31.0%) in patients with a history of surgery or transfusion.
- Sequence analysis revealed high nucleotide identity (89.14%-98.91%) between the local isolate and published HGV sequences.
Impact:
- This study provides valuable data on HGV prevalence in a Chinese population with liver disease.
- The findings suggest that HGV may not be the primary cause of non-A-E hepatitis.
- Further research is needed to fully elucidate the clinical significance and pathogenic role of HGV.
Abstract:
The aim of this article is to study the prevalence of GBV-C/HGV in patients with liver diseases and the clinical features in patients with (GBV-C/HGV infection. The serum samples were obtained from 169 patients with liver diseases in You An Hospital). Serum GBV-C/HGV RNA was detected by reverse transcription nested polymerase chain reaction (RT-nPCR) using two primer pairs of 5' untranslated region (5' UTR) of HGV. Serum anti-HGV was detected by ELISA simultaneously. The partial GBV-C/HGV genome isolated from one patient was cloned into T vectors and sequenced by dideoxy-mediated chain termination methods. Among 169 patients with various liver diseases, the GBV-C/HGV RNA positive rate was 9.5% (16/169), including 11.1% (1/9) of AHA, 4.1% (3/73) of CHB, 16.2% (6/37) of CHC, 13.3% (2/15) of AH(nonA-E) 12.5% (1/8) of CH(nonA-E) 15.4% (2/13) of LC and 33.3% (1/3) of SH cases. Of 29 patients having a history of surgical operation and transfusion, 9(31.0%) was positive for GBV-C/HGV RNA, which was remarkably higher than those cases without surgical operation and transfusion. The sequence analysis showed that there was 89.14%-98.91% nucleotide identity between our isolate and published GBV-C/HGV isolates. The results also suggested that infection rate of GBV-C/HGV was around 9.5% in patients from Beijing region, patients with GBV-C/HGV infection might show various clinical features and GBV-C/HGV might not be the major cause of hepatitis nonA-E.