Related Experiment Video
Updated: Sep 27, 2026

Semi-quantitative Detection of RNA-dependent RNA Polymerase Activity of Human Telomerase Reverse Transcriptase Protein
Published on: June 12, 2018
[The detection and clinical significance of telomerase activity in supraglottic carcinomas]
1Department of Otorhinolaryngology, the First Affiliated Hospital, China Medical University, Shenyang 110001.
Objective:
To study the expression and significance of telomerase activity in laryngeal carcinoma.
Method:
We detected telomerase activity of HEP-2 cell line, 26 cases of supraglottic carcinoma tissues and 15 cases of peri-carcinoma tissues with PCR-based assay designated TRAP (for telomeric repeat amplification protocol).
Result:
The telomerase positive rate of supraglottic carcinoma 84.6% (22/26) was obvious higher than that of peri-carcinoma 40% (6/15), P < 0.01. No significant differences in positive rates were found among different groups divided according to clinical stage or metastasis of cervical lymph node, P > 0.05.
Conclusion:
Telomerase activation is related to immortalization process, though not much helpful in diagnosis of supraglottic carcinoma.
Related Concept Videos
Telomeres and Telomerase
Replicative Cell Senescence
Mitogens and the Cell Cycle
