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Updated: Sep 27, 2026

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[Separation of saccharides in tobacco by capillary zone electrophoresis]
Qiang Ma1, You-zhao He, Xie-zhong Xiao
1Department of Chemistry, University of Science and Technology of China, Hefei 230026, China.
Abstract:
A method for the separation of derivatized saccharides in tobacco with high performance capillary electrophoresis and ultraviolet spectrophotometry (HPCE-UV) is presented. Five saccharides in tobacco were derivatized with p-aminobenzonitrile, separated at pH 10.5 with 50 mmol/L borate buffer containing 5% (volume fraction, the same in the following percentage concentration) methanol, 5% acetonitrile, 2.5% ethyleneglycol, 2.5% 2-propanol and 1 mmol/L sodium dodecyl sulfate, and detected at 285 nm.
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