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Updated: Aug 17, 2026
Isolation and Derivation of Mouse Embryonic Germinal Cells
Published on: October 22, 2009
[Establishment of mouse ZF-12+/- embryonic stem cells]
Jian-Zhong Li1, Hua Yang, Ji-Liang Fu
1Department of Medical Genetics, Second Military University, Shanghai 200433, China.
Abstract:
By using the mouse zinc finger protein gene ZF-12 genomic DNA fragment, pSSC-TV-10.5 was designed and constructed as a replacement vector. Structure of pSSC-TV-10.5 was identified by restrictive digestion analysis and partly sequencing. Then linearized vector was electroporated into ES cells, and transfected cells were screened by G418 and GANC selection. Among 508 G418r/GANCr colonies, 4 were proved to have taken place the homologous recombination of ZF-12 by PCR and southern blotting analysis. This study lays the foundations of preparing mouse models of ZF-12+/- or ZF-12-/-.
