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[Study on the effects of taxol on Hep-2 cell lines in larynx carcinoma]
Objective:
To investigate the sensitivity of Hep-2 cell lines in larynx squamous cell carcinoma to the taxol in vitro and to provide experimental basis for clinical therapy.
Method:
Techniques of cell culture and randomized blank-contrast design were used. The degree and dose-dependency of taxol which induced growth inhibition in Hep-2 cell lines were observed and the alteration of cell cycle was analysed by flow cytomethy.
Result:
All cells died at the concentration of taxol 1 x 10(-8) M; growth inhibition was observed clearly at the concentration of 5 x 10(-9) M and 2.5 x 10(-9) M. Flow cytometric data showed a G0/G1 arrest and apoptosis (28.96%) in two cell lines after exposure to taxol for 48 h at low and medium concentrations.
Conclusion:
There is a high sensitivity of Hep-2 cell to taxol in vitro. The cells show G0/G1 arrest and apoptosis. This results may provide reliable guidance for treating largnx carcinoma.
