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Experiment study of effect of perfluorohexyloctane on corneal endothelial cells
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Science, Guangzhou 510060, China.
Purpose:
To investigate the effect of Perfluorohexyloctane (F6H8) on corneal endothelial cells(CEC) of rabbit eyes.
Methods:
Fifteen New Zealand white rabbits were divided into two groups: experimental group(F6H8) and control group(BSS). All rabbits underwent anterior chamber injection of 0.15 ml F6H8 or BSS. Slit-lamp biomicroscopy and corneal endothelium photography were performed pre-operatively and postoperatively. Histopathological examination and Transmission electron microscopy(TEM) were done after the rabbits were sacrificed.
Results:
All the corneas were clear. Since 4 weeks after operation, the endothelial cells were markedly irregular in size and shape and the number of endothelial cells was markedly decreased. Multilayered retrocorneal membranes(RCM) grew gradually 2 weeks after surgery. Vacuolar degeneration was seen in some endothelial cells. Nuclear degeneration and edema of plasma were seen in TEM.
Conclusion:
Corneal endothelial cell degenerated after contacting with F6H8 for 2-4 weeks. As a silicone solvent, it should be removed completely after injection. We don't recommend it to be used as a new intraocular temponade.