[Study on tissue culture of Poligonum multiflorum]

In the present thesis, suitable medium for callus inducement and relationship between culture time and callus growth of Poligonum multiflorum were studied by mono-factorial and orthogonal design test. The experiments showed that the best medium for callus inducement was MS + 2,4-D 1 mg/L + 6-BA 1 mg/L + IBA 0.5 mg/L, dark culture was better than light culture, callus grew fast when it was cultured 12 days, whole plant could be induced by callus, the induce rate was 95%.