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Multilineage cells from adipose tissue as gene delivery vehicles
Kouki Morizono1, Daniel A De Ugarte, Min Zhu
1Microbiology, Immunology, and Molecular Genetics and Medicine, Department of Hematology-Oncology, UCLA AIDS Institute, UCLA School of Medicine, Los Angeles, CA 90024, USA.
Human Gene Therapy
|February 8, 2003
Summary
Processed lipoaspirate (PLA) cells from fat tissue show multilineage potential. These cells are efficiently transduced with lentiviral vectors, maintaining transgene expression for potential therapeutic gene delivery.
Area of Science:
- Cell Biology
- Regenerative Medicine
- Gene Therapy
Background:
- Mesenchymal progenitor cells are crucial for tissue regeneration.
- Adipose tissue offers an abundant and accessible source of these cells.
- Processed lipoaspirate (PLA) cells are a type of adipose-derived mesenchymal progenitor cell.
Purpose of the Study:
- To characterize the multilineage potential of PLA cells.
- To evaluate the efficiency of transducing PLA cells with viral vectors.
- To assess the suitability of PLA cells as a vehicle for therapeutic gene delivery.
Main Methods:
- Characterization of PLA cell multilineage differentiation potential.
- Infection of PLA cells using adenoviral, oncoretroviral, and lentiviral vectors.
- Assessment of transgene expression in PLA cells and their differentiated progeny.
Main Results:
- PLA cells exhibit multilineage potential comparable to bone marrow-derived mesenchymal stem cells.
- Lentiviral vectors efficiently transduce PLA cells.
- Transgene expression is maintained in PLA cells after differentiation into adipogenic and osteogenic lineages.
Conclusions:
- PLA cells are a promising source of mesenchymal progenitor cells.
- Lentiviral transduction of PLA cells is highly efficient.
- The combination of PLA cells and lentiviral vectors represents a potential therapeutic gene delivery strategy.