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Updated: Sep 27, 2026
Controlled Cortical Impact Model for Traumatic Brain Injury
Published on: August 5, 2014
[Effects of nao-yi-an granule on bcl-2 expression in the rat brain after experimental cerebral hemorrhage]
Hua-xian Zhang1, Xing-qun Li, Tao Tang
1Institute of Combined Traditional Chinese Medicine and Western Medicine, Xiangya Hospital, Central South University, Changsha 410008, China.
Objective:
To investigate the protective effects and mechanisms of the traditional Chinese medicine complex, nao-yi-an granule (NYA) on experimental intracerebral hemorrhage (ICH) in rats.
Methods:
ICH models anesthesized were induced by stereotaxical injection of 2 microliters normal saline containing 0.4 U bacterial collagenase(type VII) into the right globas pallidus(GP), and the protein expression of bcl-2 in the rat brain after ICH was detected with the immunohistochemistry method, with which the effects of NYA were observed.
Results:
Bcl-2 positive cells were found in the cerebral cortex and basal ganglia area after ICH. Immunoreactivity was localized in the cytoplasm as a fine granular precipitate. The levels of bcl-2 expression in the model group varied at different time-points, and reached the peak at 2 d. In the NYA group, the protein expression of bcl-2 was increased significantly both in the cortex and in the basal ganglia compared with those in the model group.
Conclusion:
NYA can significantly upregulate the protein expression of bcl-2.