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Updated: Sep 27, 2026

Growth, Purification, and Titration of Oncolytic Herpes Simplex Virus
Published on: May 13, 2021
[Experimental study on the viricidal effect of rough extracts of camptothecine fruit on herpes simplex virus-2 in
Wen-wen Li1, Zu-wei Yan, Kai Shi
1Department of Clinical Microbiology and Immunology, Xiangya School of Medicine, Central South University, Changsha 410078, China.
Objective:
To study the viricidal effect of rough extracts of camptothecine fruit on herpes simplex virus-2 (HSV-2).
Methods:
Rough extracts with different concentrations were mixed with HSV-2. The mixture was incubated at 37 degrees C for 4 h or at 4 degrees C overnight, adsorbed on monolayer cells of BGM for 1.5 h, and cultured at 37 degrees C for 7 d.
Results:
The BGM of the test group did not reveal cytopathic effects (CPE), while the control group showed CPE under microscope observation.
Conclusion:
Camptothecine has a strong viricidal effect on HSV-2.
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