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Suppression of the inflammatory response from adherent cells on phospholipid polymers
Shin-ichi Sawada1, Shujiro Sakaki, Yasuhiko Iwasaki
1Division of Organic Materials, Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda-ku, Tokyo 101-0062, Japan.
Abstract:
The expression of interleukin-1beta (IL-1beta) messenger RNA (mRNA) in macrophage-like cells cultured on phospholipid polymers was evaluated to determine the extent of the inflammatory response. As phospholipid polymers, poly(2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-butyl methacrylate(BMA)s (PMBs) were synthesized. Poly(ethylene terephthalate) (PET), poly(2-hydroxyethyl methacrylate) (PHEMA), and segmented poly(ether urethane) (Tecoflex 60) were used as reference biomedical polymers. The protein adsorption onto the polymer surfaces from a cell culture medium was determined. The amount of the total protein adsorbed onto the PMBs was lower than that adsorbed onto the reference polymers, and the amount of adsorbed protein decreased with an increase in the MPC units in the PMBs. Human premyelocytic leukemia cell line (HL-60) was used, and the expression of IL-1beta mRNA was investigated with the reverse transcription polymerase chain reaction (RT-PCR) method. When HL-60 cells were cultured on PMBs, the expression of IL-1beta mRNA in the cells was much less than that on the reference polymers. In particular, the expression of IL-1beta mRNA in HL-60 cells cultured on the PMBs containing more than 10 mol % MPC units was not detected. This corresponded to the reduced amount of adsorbed proteins on the PMB surfaces. These results suggest that the PMBs effectively suppressed the activation and inflammatory response of adherent macrophagelike cells.
Insights
New phospholipid polymers (PMBs) significantly reduce inflammatory responses in macrophage-like cells. These materials show lower protein adsorption and suppressed interleukin-1beta (IL-1beta) messenger RNA (mRNA) expression, indicating reduced cell activation.
Area of Science:
- Biomaterials Science
- Immunology
- Polymer Chemistry
Background:
- Biomedical polymers can elicit inflammatory responses when interacting with cells.
- Controlling protein adsorption on biomaterial surfaces is crucial for mitigating adverse cellular reactions.
- Phospholipid polymers offer potential for reduced inflammatory signaling.
Purpose of the Study:
- To synthesize and evaluate novel phospholipid polymers (PMBs) for their ability to suppress inflammatory responses.
- To investigate the relationship between protein adsorption and inflammatory marker expression on PMB surfaces.
- To compare the inflammatory potential of PMBs against established biomedical polymers.
Main Methods:
- Synthesis of poly(2-methacryloyloxyethyl phosphorylcholine(MPC)-co-n-butyl methacrylate(BMA)s (PMBs).
- Measurement of protein adsorption from cell culture medium onto PMBs and reference polymers (PET, HEMA, Tecoflex 60).
- Quantification of interleukin-1beta (IL-1beta) messenger RNA (mRNA) expression in HL-60 cells using reverse transcription polymerase chain reaction (RT-PCR) after culture on the polymers.
Main Results:
- PMBs exhibited lower protein adsorption compared to reference polymers.
- Protein adsorption decreased with increasing MPC content in PMBs.
- IL-1beta mRNA expression was significantly lower on PMBs, with no detectable levels on PMBs containing >10 mol% MPC.
- Reduced protein adsorption correlated with suppressed IL-1beta mRNA expression.
Conclusions:
- PMBs effectively suppress the activation and inflammatory response of adherent macrophage-like cells.
- The reduced inflammatory response is linked to lower protein adsorption on PMB surfaces.
- Phospholipid polymers represent a promising class of biomaterials for minimizing inflammatory reactions in biomedical applications.