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[Expression of bcl-2 gene in human trabecular cells induced by dexamethasone]
1Zhongshan Ophthalmic Center, Sun Yat-sen University of Medical Sciences, Guangzhou 510060, China.
Purpose:
To investigate the influence of dexamethasone on expression of bcl-2 gene in human trabecular cells.
Methods:
The trabecular specimens from human eyes were cultured in vitro and the cultured cells of the third generation on slides were immersed in culture solution containing 10(-7) M dexamethasone for 6 hours, 12 hours and 24 hours respectively. The expression of bcl-2 gene was studied with LSAB.
Results:
The positive cells of the bcl-2 protein could be identified after dexamethasone effected for 6 hours and the positive cell increased with time going on.
Conclusion:
The expression of bcl-2 gene can be induced by dexamethasone, it may be related with glaucomatic pathogenesis.

