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[Determination of irisquinone by single sweep oscillopolarography]
Ping He1, Jun-feng Song, Wei Guo
1Institute of Analytical Science, Northwest University, Xi'an 710069, China.
Aim:
To propose a polarographic method for the determination of irisquinone.
Methods:
A reduction wave of irisquinone was recorded by single sweep oscillopolarography.
Results:
In 8.0 x 10(-3) mol.L-1 Na2B4O7-1.6 x 10(-2) mol.L-1 KH2PO4 (pH 7.7) supporting electrolyte, a redution wave of irisquinone with peak potential -1.23 V (vs SCE) achieved high sensitivity. The 2nd-order derivative peak current of the reduction wave was proportional to irisquinone concentration in the range of 1.5 x 10(-7)-5.2 x 10(-6) mol.L-1 (gamma = 0.9992, n = 9). The detection limit was 6.0 x 10(-8) mol.L-1. Relative standard deviation (RSD) was 0.87% by performing 13 independent measurements on 2.0 x 10(-6) mol.L-1 irisquinone.
Conclusion:
The proposed method was sensitive, simple, rapid, and can be applied to the determination of irisquinone in raw medicine and capsule.