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[Morphological changes of cultured rabbit retinal cells after injury]
1Dept. of Ophthalmology, Xijing Hospital, 4th Military Medical University, XiAn 710032, China.
Purpose:
To investigate the morphological changes of cultured rabbit retinal cells after injury.
Methods:
Mechanical scratch was done in nenatol rabbit retinal cell culture and in Schwann Cell(SC)/retinal cell colculture. The morphological changes of different sorts of cells after the injury were observed under the phase contrast microscope for 24 h.
Results:
About 30 min after injury, many glial cells moved to the blank region and proliferated to form gliosis. Axons of retinal neurons began to regenerate 6 h post injury. The direction of regrowth was influenced by the environment. In the coculture, regeneration of axons was discerned only 3 h after injury.
Conclusion:
Axons of retinal neurons had strong capability of regeneration; Gliosis was not the absolute obstacle of regeneration; SC played a role in promoting the axon regeneration.