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[Campylobacter jejuni and C. coli in aborted swine: comparison between phenotypic identification and polyacrylamide
G I Giacoboni1, M G Echeverría, C J Perfumo
1Laboratorio de Diagnóstico e Investigaciones Bacteriológicas, Universidad Nacional de La Plata, 60 y 118, CC 296, La Plata B1900AVW, Argentina. giacoboni@fcv.unlp.edu.ar
Abstract:
Campylobacter jejuni and Campylobacter coli were isolated from aborted pig fetuses which proceeded from different animals and farms between February 2000 and March 2001. Seven Campylobacter jejuni biotype II, three biotype I and one Campylobacter coli biotype I were identified by phenotypic tests and Lior's scheme. To corroborate and compare the phenotypic results, 7.5, 10 and 12.5% polyacrilamide gel electrophoresis (SDS-PAGE) were used under reducing conditions. Characteristic bands of hypervariable dense zone within C. jejuni and C. coli species were observed in all the whole cell protein extracts with differences in mobility. It was possible to establish differences between identical phenotypic Campylobacter isolates and different protein profile from fetuses of the same litter. SDS-PAGE is a stable and reproducible method to establish differences between Campylobacter strains and is considered applicable for the differentiation of the wide variability of Campylobacter species for epidemiologic purposes.