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Platelet dense bodies: a quantitative microprobe analysis.

R J Skaer, J P Emmines, P D Peters

    Journal of Cell Science
    |March 1, 1976
    PubMed
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    Human platelet dense bodies contain significant calcium and phosphorus. Electron microprobe analysis quantified these elements, revealing their atomic ratios and amounts, crucial for understanding platelet function.

    Area of Science:

    • Biochemistry
    • Hematology
    • Analytical Chemistry

    Background:

    • Platelet dense bodies are critical storage sites for various molecules.
    • The precise elemental composition of dense bodies, particularly calcium and phosphorus, is not fully elucidated.
    • Understanding dense body composition is key to platelet aggregation mechanisms.

    Purpose of the Study:

    • To quantify the atomic ratios and absolute amounts of calcium and phosphorus in human platelet dense bodies.
    • To investigate the role of lipids in dense body composition.
    • To compare dense body composition in human and pig platelets.

    Main Methods:

    • Electron microprobe analysis was used to determine phosphorus:calcium (P:Ca) peak ratios.
    • Lipid extraction using chloroform/methanol was performed to assess lipid-bound phosphorus.

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  • Calibration with calcium and phosphorus standards allowed conversion to atomic ratios and absolute quantification.

    • Main Results:

    • Human platelet dense bodies exhibit a mean P:Ca peak ratio of 1-2, decreasing to 0.89 after lipid extraction.
    • The mean atomic P:Ca ratio was determined to be 1.76.
    • Calculated calcium content is 0.6 mg/10(11) platelets (2.97 mg Ca/g dry weight), within the known range for total platelet calcium.
    • Pig platelet dense bodies contain both magnesium and calcium in variable ratios.

    Conclusions:

    • Dense bodies contain substantial amounts of calcium and phosphorus, with a significant portion associated with non-lipid components.
    • The findings provide quantitative data on dense body elemental composition, contributing to the understanding of platelet physiology.
    • The elemental composition may play a role in regulating platelet aggregation.