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Related Experiment Videos

Measuring lung function in mice: the phenotyping uncertainty principle.

Jason H T Bates1, Charles G Irvin

  • 1Vermont Lung Center and College of Medicine, University of Vermont, Burlington, Vermont 05405, USA. jhbates@zoo.uvm.edu

Journal of Applied Physiology (Bethesda, Md. : 1985)
|March 11, 2003
PubMed
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Assessing mouse lung function is key for human disease models. This review introduces the phenotyping uncertainty principle, balancing noninvasiveness with measurement precision for accurate lung function assessment in mice.

Area of Science:

  • Pulmonary physiology
  • Animal modeling
  • Respiratory mechanics

Background:

  • Accurate measurement of mouse lung function is critical for validating murine models of human lung disease.
  • Technical challenges arise from the small size of mice, particularly in measuring respiratory airflow.

Purpose of the Study:

  • To review and contrast existing methods for assessing lung function in mice.
  • To introduce the "phenotyping uncertainty principle" to evaluate these methods.
  • To guide the selection of appropriate techniques based on experimental needs.

Main Methods:

  • The review examines various lung function assessment methods in mice.
  • Methods are contrasted based on a trade-off between noninvasiveness and experimental control/precision.

Related Experiment Videos

  • Key examples discussed include unrestrained plethysmography, input impedance, and transfer impedance.

    • Main Results:

    • Unrestrained plethysmography offers high noninvasiveness but lacks meaningful links to respiratory mechanics.
    • Input impedance measurement provides high precision but requires highly invasive conditions (anesthesia, paralysis, tracheostomy).
    • Transfer impedance in conscious, restrained mice offers a balance between invasiveness and precision.

    Conclusions:

    • No single method for mouse lung function assessment is universally optimal.
    • The choice of method depends on the specific research application and the required balance between invasiveness and precision.
    • Understanding the phenotyping uncertainty principle aids in selecting the most suitable technique.