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Microfiltration and ultrafiltration of polysaccharides produced by fermentation using a rotating disk dynamic
A Brou1, M Y Jaffrin, L H Ding
1Department of Biological Engineering, UMR CNRS 6600, University of Technology of Compiegne, BP 20529, 60205 Compiegne Cedex, France.
Biotechnology and Bioengineering
|March 13, 2003
Summary
This study optimized exopolysaccharide (EPS) recovery using a novel rotating disk microfiltration system with vanes. The enhanced design significantly increased permeate flux and reduced energy consumption for efficient EPS production.
Area of Science:
- Biotechnology
- Separation Science
- Microbial Fermentation
Background:
- Exopolysaccharides (EPS) are crucial biopolymers with diverse applications.
- Efficient recovery methods are needed to enhance the economic viability of EPS production.
- Sinorhizobium meliloti is a key bacterium for EPS synthesis.
Purpose of the Study:
- To investigate the recovery of exopolysaccharides (EPS) produced by Sinorhizobium meliloti using dynamic microfiltration.
- To evaluate the performance of a novel rotating disk device with vanes for enhanced permeate flux and EPS transmission.
- To assess the energy efficiency of the developed system for EPS concentration via ultrafiltration.
Main Methods:
- Utilized a laboratory-designed rotating disk device with a 0.2 microm nylon membrane.
- Compared filtration performance with and without vanes on the rotating disk.
- Investigated the effect of operating parameters such as rotation speed, vane geometry, and broth temperature.
- Employed ultrafiltration with a 50 kDa PES membrane for EPS concentration.
- Measured net electrical power consumption to determine specific energy usage.
Main Results:
- The rotating disk device with vanes significantly increased permeate flux compared to a smooth disk.
- A 6-mm vanes disk at 3000 rpm and 36 degrees C achieved a maximum flux of 1560 g h(-1) m(-2).
- Permeate flux correlated with membrane shear stress, indicating vanes enhance fluid core velocity.
- Ultrafiltration achieved high EPS rejection (nearly complete) at 160 L h(-1) m(-2).
- The system with 6-mm vanes demonstrated minimal specific energy consumption per cubic meter of permeate.
Conclusions:
- The novel rotating disk microfiltration system with vanes offers superior performance for EPS recovery.
- The design enhances permeate flux and EPS transmission while maintaining high concentration efficiency.
- This technology presents a more energy-efficient approach for industrial-scale exopolysaccharide production and recovery.