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[Performance study of analytical method for ethoxyquin in fruit].

Kumiko Sasaki1, Takashi Tatsuno, Takakazu Nomura

  • 1National Institute of Health Sciences: 1-18-1, Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan.

Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
|March 15, 2003
PubMed
Summary

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A new analytical method for ethoxyquin (ET) in fruits shows improved extraction efficiency using acetone. This validated method offers reliable detection limits for ensuring food safety.

Area of Science:

  • Food Chemistry
  • Analytical Chemistry
  • Agricultural Science

Context:

  • Ethoxyquin (ET) is a widely used antioxidant in food production.
  • Official analytical methods for ET determination may have limitations in extraction efficiency.
  • Accurate quantification of ET is crucial for food safety and regulatory compliance.

Purpose:

  • To develop and validate a novel analytical method for ethoxyquin (ET) determination in apples and pears.
  • To enhance extraction efficiency and prevent decomposition of ET during analysis.
  • To evaluate the method's performance through a multi-laboratory study.

Summary:

  • A modified analytical method using thiourea homogenization and acetone extraction for ethoxyquin (ET) was developed.
  • Dibutyl hydroxytoluene was added to prevent ET decomposition, improving accuracy.

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  • Multi-laboratory validation demonstrated good mean recoveries (83.0–85.3%) and acceptable repeatability (3.8–4.7% RSD) and reproducibility (7.8–11.3% RSD).
  • Low detection limits (0.001–0.025 µg/g) were achieved, with ET successfully detected by LC/MS and GC/MS.

    • Impact:

    • Provides a more efficient and reliable method for ethoxyquin analysis in fruits.
    • Contributes to enhanced food safety monitoring and quality control.
    • Supports regulatory bodies in enforcing standards for ethoxyquin residues.