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Updated: Feb 1, 2026

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice
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Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

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Proteomic analysis of rat kidney cortex following treatment with gentamicin

Joanne Charlwood1, J Mark Skehel, Nick King

  • 1GlaxoSmithKline, New Frontiers Science Park, Third Avenue, Harlow, Essex CM19 5AW, UK.

Journal of Proteome Research
|March 20, 2003
PubMed

Insights

Gentamicin exposure damages kidneys by affecting cellular energy production and causing mitochondrial dysfunction. This study identifies over 20 proteins involved in key metabolic pathways disrupted by this nephrotoxic antibiotic.

Area of Science:

  • Nephrology
  • Toxicology
  • Proteomics

Background:

  • Kidney's regional structure makes it vulnerable to toxins.
  • Gentamicin is an antibiotic known for potential kidney damage.

Purpose of the Study:

  • To investigate gentamicin's effects on kidney proteome.
  • To characterize protein expression changes in response to gentamicin.

Main Methods:

  • Proteomic analysis of kidney tissue.
  • Quantification of protein expression levels after gentamicin treatment.

Main Results:

  • Over 20 proteins were identified with altered expression.
  • Affected proteins are involved in energy metabolism (citric acid cycle, gluconeogenesis), fatty acid synthesis, transport, and cellular stress.
  • Evidence of impaired energy production and mitochondrial dysfunction.

Conclusions:

  • Gentamicin-induced nephrotoxicity involves significant disruption of cellular energy pathways.
  • Mitochondrial dysfunction is a key mechanism in gentamicin's kidney damage.
  • Proteomic insights reveal molecular targets of gentamicin toxicity.

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