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Comparative studies on algal toxicity testing using fluorometric microplate and Erlenmeyer flask growth-inhibition
Adolf Eisentraeger1, Wolfgang Dott, Joern Klein
1Medical Faculty of the RWTH Aachen, Institute of Hygiene and Environmental Medicine, Pauwelsstrasse 30, D-52074 Aachen, Germany. adolf.eisentraeger@post.rwth-aachen.de
Abstract:
Two fluorometric microplate algae growth-inhibition assays with a liquid volume of 2 mL and 200 microL per well are presented, and comparative studies on the toxicity of chemicals are carried out with Erlenmeyer flask assays. The test procedures are in accordance with the standards ISO 8692 (DIN 38412 L9 and EN 28692), OECD 201 and DIN 38412 L33. By testing four toxicants several times laboratory internal repeatability is proven. Statistical evaluation demonstrates that the results obtained with both the 24-well and the 96-well microplates are nearly identical with the results of the Erlenmeyer flask assay. Therefore, the microplate growth-inhibition assays can be applied for the testing of a wide range of chemicals and environmental samples if some methodical aspects are taken into account. Apart from that, there is a strong need for harmonization if the dependency of the EC values on the toxicological endpoint is considered.