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Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging
Michael Speidel1, Alexandr Jonás, Ernst-Ludwig Florin
1Cell Biology and Biophysics Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany.
Optics Letters
|March 27, 2003
Abstract:
We show that the position of a fluorescent nanoparticle can be measured in three dimensions with subnanometer precision and 100-ms temporal resolution by use of standard epifluorescence video imaging in off-focus mode. The particle can be tracked without feedback in a volume of at least 40 microm x 60 microm x 3 microm. With the technique presented, the structure-mobility relationship of 216-nm latex particle in a porous polymer network was studied in three dimensions.