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Maturation of Endosomes

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The early endosome containing internalized molecules matures through transformations in its location, morphology, intraluminal pH, and membrane protein composition. Together, these changes result in a more acidic late endosome that contains multiple intraluminal vesicles; therefore, the late endosome is also called a multivesicular body (MVB).
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Apoptosis

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Related Experiment Video

Updated: Jun 28, 2026

Detection and Isolation of Apoptotic Bodies to High Purity

Detection and Isolation of Apoptotic Bodies to High Purity

Published on: August 12, 2018

Proteomic approach to apoptotic thymus maturation.

Takao Kawakami¹, Takuya Nagata, Atsushi Muraguchi

¹Chemistry Department, Research Division, GlaxoSmithKline KK, 43 Wadai, Tsukuba-shi, Ibaraki 300-4247, Japan.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|March 28, 2003

Summary

Apoptosis, crucial for T lymphocyte selection during thymus maturation, can be systematically studied using proteomics. This approach analyzes protein expression profiles to understand cell death pathways.

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Area of Science:

Immunology
Molecular Biology
Biochemistry

Background:

Apoptosis is vital for T lymphocyte selection in mammalian thymus maturation.
Understanding the molecular mechanisms of apoptosis is key to immune system development.

Purpose of the Study:

To highlight the utility of proteomics in systematically analyzing protein molecules involved in apoptosis.
To demonstrate how protein expression profiles can aid in constructing intracellular pathway models of apoptotic cell death.

Main Methods:

Proteomics offers a comprehensive approach to study cellular protein expression.
Analysis of protein signatures, localization, and quantitative changes is employed.

Main Results:

Protein expression profiles provide insights into the complex biological system of apoptosis.
Quantitative changes in proteins in response to extracellular stimuli are identified.

Conclusions:

Proteomics is a powerful tool for dissecting the molecular intricacies of apoptosis.
Detailed protein information facilitates the modeling of intracellular pathways leading to programmed cell death.