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[Collagenase activity of Streptomyces sp. 1349]
O V Ivanko1, L D Varbanets', O V Valahurova
1Institute of Microbiology and Virology, National Academy of Sciences of Ukraine, 154 Zabolotny St., Kyiv, 03143, Ukraine.
Abstract:
Streptomyces sp. 1349 is the active producer of collagenase. The dynamics of growth, influence of pH and temperature on collagenase activity and heterogeneity of population were studied. Maxima of the enzyme activity, proteins and biomass reach their highest point on the 3-4th day. The complex of enzymes obtained from culture liquid has pH-optimum of collagenase activity in neutral (pH 6-7) and alkaline (pH 9-10) regions, and thermooptimum at 37 and 60 degrees C. The preparation is stable. Four types of colonies with different level of collagenase activity were revealed during analysis of the heterogeneity of Streptomyces sp. 1349 population. The variant of basic type was selected for further work with activity that exceeds 2-3 times that of the control.