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Updated: Sep 26, 2026
DNA Vector-based RNA Interference to Study Gene Function in Cancer
Published on: June 4, 2012
[Inhibition of Luciferase expression in mammalian cells by AAV vector plasmid mediated Luciferase shRNA]
Xin Ma1, Xiaochun Lu, Jainqiang Peng
1Institute of Virology,Chinese Academy of Preventive Medicine, Beijing 100052, China.
Objective:
Constructing a plasmid containing the shRNA of luciferase to suppress the expression of luciferase in BHK-21 cell.
Methods:
A 334 bp human U6 snRNA promoter was amplified from human genomic DNA by PCR and ligated to a 21 bp reverse repeated motif of luciferase target sequence with 9 bp spacer and AAV plasmid pSNAV. The recombinant pSNAV/U6/Luc plasmid cotransfected with pMAMneoLuc or transfected luciferase cell line to detect the effect of luciferase expression separately.
Results:
pSNAV/U6/luc suppresses the luciferase expression from pMAMneoLuc by 50% and luciferase cell line by 70%.
Conclusions:
The results showed that the short hairpin RNA of luciferase can efficiently suppress its expression in BHK-21.
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