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Updated: Sep 26, 2026
Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α
Published on: June 14, 2018
[Preparation and application of monoclonal antibodies to recombinant human IFN alpha]
Meiying Wu1, Yanping Ai, Ren Ren
1Beijing Tri-prime Genetic Engineering Co. Ltd, Beijing 102600, China.
Objective:
To prepare and apply monoclonal antibodies (McAb) against recombinant human interferon alpha (rHu IFN-alpha).
Methods:
Five cell lines (2E9, 4G1, 2A7, 2C9, 4G10) secreting McAbs against rHu IFN-alpha were established by hybridoma technique.
Results:
All the cell lines secreted monoclonal antibodies stably. Functions of secreting antibodies of the five cell lines lasted for 6 months in BALB/c mice and 8 months in cell culture. The specificity of antibody was constant. The Ig subclasses of the McAbs were IgG1. Anti-IFN McAb affinity purification column was prepared by coupling the anti IFN-alpha McAb to Sepharose 4B. The combining rate reached was higher than 95%.
Conclusions:
The highest purification efficiency was obtained by using 4G10 column.
