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An improved ferritin assay for canine sera.
Gordon A. Andrews1, Joseph E. Smith, Mary Gray
1Departments of Pathology and Microbiology, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506-5605.
Veterinary Clinical Pathology
|January 1, 1992
Summary
A new canine serum ferritin assay was developed using monoclonal antibodies. This assay accurately measures serum ferritin in dogs, with a geometric mean of 252 ng/ml in healthy individuals.
Area of Science:
- Veterinary diagnostics
- Immunochemistry
- Canine health
Background:
- Serum ferritin is a key indicator of iron status and inflammation in animals.
- Existing assays for canine serum ferritin may have limitations in accuracy or sample requirements.
Purpose of the Study:
- To develop and validate an improved assay for quantifying serum ferritin in dogs.
- To establish reference ranges for serum ferritin in healthy canines.
Main Methods:
- Development of a sandwich immunoassay utilizing two monoclonal antibodies.
- Assay validation including recovery, within-assay, and assay-to-assay variability.
- Analysis of serum ferritin levels in a cohort of 61 healthy dogs.
Main Results:
- The improved assay demonstrated high recovery rates (98-106%) for added ferritin.
- Excellent precision was observed with within-assay variability of 3.3-4.5% and assay-to-assay variability of 9.8-10.2%.
- The geometric mean serum ferritin concentration in healthy dogs was 252 ng/ml (range: 80-800 ng/ml).
Conclusions:
- A robust and reliable immunoassay for canine serum ferritin has been successfully developed.
- This assay allows for accurate measurement of serum ferritin in undiluted canine serum samples.
- The established reference range provides a valuable benchmark for assessing iron status and health in dogs.