Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Alkylphospholipids reversibly open epithelial tight junctions.

Ancy Leroy1, Georges K P de Bruyne, Lauran C J M Oomen

  • 1Laboratory of Experimental Cancerology, Ghent University Hospital, De Pintelaan, 185, B-9000 Ghent, Belgium.

Anticancer Research
|April 12, 2003
PubMed
Summary

Alkylphospholipids rapidly decrease transepithelial electrical resistance in colorectal cancer cells, indicating altered tight junction integrity. This effect is reversible and suggests alkylphospholipids impact cell-cell adhesion complexes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chick Heart Invasion Assay for Testing the Invasiveness of Cancer Cells and the Activity of Potentially Anti-invasive Compounds.

Journal of visualized experiments : JoVE·2015
Same author

Chick heart invasion assay.

Methods in molecular biology (Clifton, N.J.)·2013
Same author

Opioids affect focal contact-mediated cell-substrate adhesion.

European journal of cancer prevention : the official journal of the European Cancer Prevention Organisation (ECP)·2010
Same author

Direct effects of delta opioid receptor agonists on invasion-associated activities of HCT-8/E11 colon cancer cells.

Anticancer research·2010
Same author

Helicobacter pylori induces gastric epithelial cell invasion in a c-Met and type IV secretion system-dependent manner.

The Journal of biological chemistry·2006
Same author

Colon cancer cells: pro-invasive signalling.

The international journal of biochemistry & cell biology·2006

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Cancer Research

Background:

  • Alkylphospholipids, like 1-O-octadecyl-2-O-methylglycero-3-phosphocholine, are known to modulate cancer cell invasion by affecting adherens junctions.
  • Epithelial cells are organized by cell-cell adhesion complexes, including adherens junctions and tight junctions, which maintain tissue integrity.

Purpose of the Study:

  • To investigate the effect of alkylphospholipids on the integrity of tight junctions in polarized epithelial cells.
  • To determine if alkylphospholipids alter the functional properties of tight junctions in human colorectal cancer cells.

Main Methods:

  • Human colorectal cancer cell layers (T84) were cultured in two-compartment chambers.
  • Transepithelial electrical resistance (TER) was measured to assess tight junction functional integrity.

Related Experiment Videos

  • Changes in protein solubility and lipid domain interactions were analyzed.

    • Main Results:

    • Incorporation of alkylphospholipids led to a rapid, reversible decrease in transepithelial electrical resistance.
    • This decrease correlated with increased paracellular permeability and was independent of temperature.
    • Alkylphospholipids altered the detergent-solubility of zonula occludens-protein and occludin, but did not specifically displace lipids from raft-like membrane domains.

    Conclusions:

    • Non-toxic doses of alkylphospholipids affect tight junction integrity in colorectal cancer cells.
    • Alkylphospholipids likely impact multiple cell-cell adhesion complexes by integrating into the plasma membrane lipid bilayer.
    • Further research is warranted to elucidate the precise molecular mechanisms involved.