A zero-crossing technique for the multidetermination of thiamine HCl and pyridoxine HCl in their mixture by using...

A new zero-crossing technique based on one-dimensional wavelet transform (WT) was developed and applied on a commercial vitamin product and binary mixtures containing thiamine HCl and pyridoxine HCl in the presence of the interference of the analysed signals. We selected from the data of the UV-Vis absorption spectra a signal consisting of 1150 points corresponding to the concentration range 8-32 mg/ml for both vitamins and we subjected it to one-dimensional continuous WT Mexican (MEXICAN) and Meyer (MEYER). Since the peaks of the transformed signals were bigger than original ones a zero crossing technique was applied to obtain the regression equations. The validity of Beer-Lambert law was assumed for the transformed signals. An appropriate scale setting was choosing to obtain an alternative calibration for each method. The basic concepts about wavelet method were briefly explained and MATLAB 6.5 software was used for one-dimensional wavelet analysis. The obtained results were successfully compared among each other and with those obtained by other literature methods. The developed method is rapid, easy to apply, not expensive and suitable for analysing of the overlapping signals of compounds in their mixtures without any chemical pre-treatment.