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Updated: Aug 4, 2026

Use of a High-throughput In Vitro Microfluidic System to Develop Oral Multi-species Biofilms
Published on: December 1, 2014
Monitoring biofilm formation and activity in drinking water distribution networks under oligotrophic conditions
R Boe-Hansen1, A C Martiny, E Arvin
1Environment and Resources DTU, Building 115, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark.
Abstract:
In this study, the construction a model distribution system suitable for studies of attached and suspended microbial consisted of two loops connected in series with a total of 140 biofilm sampling points. The biofilm from the system was studied using 11 different microbial methods and the results were compared and discussed. The methods were used for biomass quantification (AODC, HPC and ATP determination), visualisation of structure (CLSM), activity measurement (leucine incorporation, AOC removal rate, respiration of benzoic acid, CTC and live/dead stains), and microbial diversity profiling (clone libraries and DGGE).
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