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Related Experiment Video

Updated: Jan 20, 2026

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Monitoring of drinking water quality using automated ATP quantification.

C B Hansen1, A Kerrouche2, K Tatari1

  • 1Technical University of Denmark, Lyngby, Denmark.

Journal of Microbiological Methods
|September 3, 2019
PubMed
Summary

A new automated microfluidic system rapidly detects drinking water contamination using microbial Adrenosine-5'-Triphosphate (ATP) as a bacterial indicator. This technology shows promise for real-time water quality monitoring.

Keywords:
ATPDrinking waterMicrobial contaminationMicrofluidicsMonitoring

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Area of Science:

  • Environmental Science
  • Analytical Chemistry
  • Biotechnology

Background:

  • Drinking water contamination poses significant public health risks.
  • Rapid and reliable detection methods are crucial for water quality monitoring.
  • Microbial Adrenosine-5 -Triphosphate (ATP) serves as a sensitive indicator of bacterial presence.

Purpose of the Study:

  • To develop an automated microfluidic system for rapid detection of drinking water contamination.
  • To utilize microbial ATP as a key indicator for bacterial contamination.
  • To report the design, integration, and operational parameters of the developed system.

Main Methods:

  • Development of a polymethyl methacrylate microfluidic cartridge.
  • Integration of fluid storage, delivery, lysis, and real-time detection functionalities.
  • Optimization of lysis method, mixing circuit, flow rate, temperature, and reagent concentration.

Main Results:

  • Demonstrated highly linear calibration curves for total and free ATP over a wide concentration range (2.5-5000 pg/mL).
  • Achieved a limit of detection lower than 2.5 pg/mL for total ATP.
  • Observed lysis efficiency dependent on water type during laboratory trials.

Conclusions:

  • The developed microfluidic system offers a promising approach for automated, rapid detection of bacterial contamination in drinking water.
  • Further research and development are necessary to enable successful online implementation.
  • The system's performance is influenced by water matrix effects, requiring further investigation.