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Updated: Sep 26, 2026
A Robust Discovery Platform for the Identification of Novel Mediators of Melanoma Metastasis
Published on: March 8, 2022
[Anti-invasive and anti-metastatic effect of ampelopsin on melanoma]
1Department of Chemistry, Sun Yat-sen Medical College, Sun Yat-sen University, Guangzhou, Guangdong, PR China.
Background & Objective:
The authors had firstly reported that ampelopsin possess anticancer effects on several human cancer cell lines in vitro and on transplant mouse B16 melanoma in vivo. In order to further explore its antitumor effect, the authors designed this study to investigate the effect of ampelopsin on invasion and metastasis of B16 melanoma in vivo and in vitro.
Methods:
B16 cells were injected into C57BL/6 mouse via tail lateral vein, and subsequently formed an experimental pulmonary metastasis. Ampelopsin was administered at 3 dosages by intraperitoneal injection daily for 18 days from the day before cell injection. The B16 mouse melanoma cells were treated with ampelopsin for 3 days. The effect of ampelopsin on invasion, migration,and adhesion of B16 melanoma cells were evaluated using Transwell chambers attached with polycarbonate filters and reconstituted basement membrane (Matrigel).
Results:
The number of metastases in the mice that were given ampelopsin at the dosages of 150, 200, and 250 mg/kg significantly reduced as compared to the control (P< 0.05), and the inhibition rates were 30.97%, 40.58%, and 61.16%, respectively. The ability of the ampelopsin treated B16 cells to invade the reconstituted basement membrane decreased significantly (P< 0.01), and the inhibition rates were 36.06%, 59.58%, and 79.09% for ampelopsin at 20, 40, and 80 micromol/L, respectively. Ampelopsin can also inhibit B16 cells migration,and the inhibition rates were 51.59%, 56.51%, and 66.75% for ampelopsin at 20, 40, and 80 micromol/L, respectively (P< 0.01). The ability of adhesion of the B16 cells with fibronectin, laminin, or Matrigel decreased significantly.
Conclusion:
Ampelopsin has anti-invasive and anti-metastatic effects on B16 melanoma.
Insights
Ampelopsin significantly reduces B16 melanoma metastasis and invasion in mice and melanoma cells. This natural compound shows potent anti-metastatic effects, inhibiting tumor spread and cell migration.
Area of Science:
- Pharmacology
- Oncology
- Biochemistry
Context:
- Previous research indicated ampelopsin's anticancer properties against various cancer cell lines and B16 melanoma in vivo.
- Further investigation is warranted to understand ampelopsin's specific mechanisms against melanoma invasion and metastasis.
Purpose:
- To evaluate the anti-invasive and anti-metastatic effects of ampelopsin on B16 melanoma.
- To assess the impact of ampelopsin on melanoma cell invasion, migration, and adhesion in vitro and in vivo.
Summary:
- Ampelopsin administration in mice significantly reduced pulmonary metastases in a dose-dependent manner (inhibition rates up to 61.16%).
- In vitro, ampelopsin treatment markedly decreased B16 melanoma cell invasion (up to 79.09%) and migration (up to 66.75%).
- Ampelopsin also significantly inhibited the adhesion of B16 cells to extracellular matrix components like fibronectin, laminin, and Matrigel.
Impact:
- Ampelopsin demonstrates significant anti-invasive and anti-metastatic potential against B16 melanoma.
- These findings suggest ampelopsin as a potential therapeutic agent for preventing melanoma metastasis.
