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Centrosome hyperamplification and chromosomal instability in bladder cancer
K Kawamura1, M Moriyama, N Shiba
1Department of Urology, Kanazawa Medical University, 1-1 Daigaku Uchinada, 920-0293, Ishikawa, Japan. kawamura@kanazawa-med.ac.jp
European Urology
|April 23, 2003
Summary
Centrosome hyperamplification (CH) is common in bladder cancer and linked to chromosomal instability (CIN). High-grade tumors show increased CH and CIN, suggesting CH contributes to CIN development in bladder cancer.
Area of Science:
- Oncology
- Cell Biology
- Genetics
Background:
- Chromosomal instability (CIN) is a hallmark of malignant tumors.
- Centrosome hyperamplification (CH) is frequently observed in human cancers and may drive CIN.
Purpose of the Study:
- To investigate the relationship between centrosome hyperamplification (CH) and chromosomal instability (CIN) in bladder cancer.
- To analyze the centrosome replication cycle in relation to tumor grade and CIN.
Main Methods:
- Examined 22 bladder cancer samples (grades G1-G3) using immunohistochemistry for CH and FISH for CIN.
- Analyzed centrosome replication cycle using laser scanning cytometry (LSC).
Main Results:
- 81.8% of bladder cancer cases exhibited CH, with higher grades correlating with tumor histological grade (p=0.03).
- Pathologically high-grade bladder cancers showed marked centrosome variability and CIN.
- Both CH and CIN were detected in high-grade tumors, with CH grade directly proportional to CIN grade (p=0.0079).
Conclusions:
- Centrosome hyperamplification (CH) is strongly associated with chromosomal instability (CIN) in bladder cancer.
- CH may play a significant role in the development of CIN in bladder cancer.