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Related Experiment Videos

Centrosome hyperamplification and chromosomal instability in bladder cancer.

K Kawamura1, M Moriyama, N Shiba

  • 1Department of Urology, Kanazawa Medical University, 1-1 Daigaku Uchinada, 920-0293, Ishikawa, Japan. kawamura@kanazawa-med.ac.jp

European Urology
|April 23, 2003
PubMed
Summary

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Centrosome hyperamplification (CH) is common in bladder cancer and linked to chromosomal instability (CIN). High-grade tumors show increased CH and CIN, suggesting CH contributes to CIN development in bladder cancer.

Area of Science:

  • Oncology
  • Cell Biology
  • Genetics

Background:

  • Chromosomal instability (CIN) is a hallmark of malignant tumors.
  • Centrosome hyperamplification (CH) is frequently observed in human cancers and may drive CIN.

Purpose of the Study:

  • To investigate the relationship between centrosome hyperamplification (CH) and chromosomal instability (CIN) in bladder cancer.
  • To analyze the centrosome replication cycle in relation to tumor grade and CIN.

Main Methods:

  • Examined 22 bladder cancer samples (grades G1-G3) using immunohistochemistry for CH and FISH for CIN.
  • Analyzed centrosome replication cycle using laser scanning cytometry (LSC).

Main Results:

  • 81.8% of bladder cancer cases exhibited CH, with higher grades correlating with tumor histological grade (p=0.03).

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  • Pathologically high-grade bladder cancers showed marked centrosome variability and CIN.
  • Both CH and CIN were detected in high-grade tumors, with CH grade directly proportional to CIN grade (p=0.0079).

    • Conclusions:

    • Centrosome hyperamplification (CH) is strongly associated with chromosomal instability (CIN) in bladder cancer.
    • CH may play a significant role in the development of CIN in bladder cancer.