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Microdevice with integrated dialysis probe and biosensor array for continuous multi-analyte monitoring.
1Immunoassay Laboratory, I.R.-R.P., N.C.S.R Demokritos, 15310, Athens, Greece.
Biosensors & Bioelectronics
|April 23, 2003
Summary
This study presents a novel microdevice for simultaneous on-line glucose and lactate monitoring. The biosensor array demonstrates high accuracy and stability, suitable for continuous clinical analysis.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biosensor Technology
Background:
- Accurate and simultaneous monitoring of glucose and lactate is crucial for clinical diagnostics and metabolic studies.
- Existing methods often lack the real-time capabilities required for continuous physiological monitoring.
Purpose of the Study:
- To develop and evaluate a microdevice for simultaneous on-line determination of glucose and lactate.
- To assess the performance, stability, and potential interferences of the developed biosensor array.
Main Methods:
- Integration of a dialysis sampling system with a microfabricated biosensor array.
- Utilized stacked dry resist layers on a plastic support for fluidic and electric connections.
- In vitro performance evaluation, including cross-talk analysis, recovery tests, and interference studies.
Main Results:
- Negligible cross-talk between sensors was observed.
- Achieved over 95% recovery for both glucose and lactate at perfusion rates <= 0.5 microl/min.
- Demonstrated a response time of 2.4 min, linear ranges up to 30 mM (glucose) and 15 mM (lactate), and minimal interference from common electroactive species.
Conclusions:
- The developed microdevice enables reliable simultaneous on-line monitoring of glucose and lactate.
- The biosensor array exhibits excellent stability and accuracy, making it suitable for continuous analysis in buffer and serum samples.