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Antioxidative compounds from Crotalaria sessiliflora
Abdul Mun'im1, Osamu Negishi, Tetsuo Ozawa
1Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan.
Bioscience, Biotechnology, and Biochemistry
|May 6, 2003
Summary
Seven antioxidative compounds were isolated from C. sessiliflora. Hydroxyeucomic acid demonstrated potent free radical scavenging, while orientin and isoorientin offered significant anti-peroxidative and radical protective effects.
Area of Science:
- Phytochemistry
- Natural Products Chemistry
- Pharmacognosy
Background:
- Plant extracts are a rich source of bioactive compounds.
- Antioxidants play a crucial role in combating oxidative stress.
- C. sessiliflora (tanukimame) is a plant with potential medicinal properties.
Purpose of the Study:
- To isolate and identify antioxidative compounds from C. sessiliflora.
- To evaluate the free radical scavenging and anti-peroxidative activities of isolated compounds.
- To compare the efficacy of these compounds with known antioxidants.
Main Methods:
- Activity-guided fractionation of the EtOAc extract of C. sessiliflora aerial parts.
- Utilized 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay for fractionation.
- Assessed anti-peroxidative activity against linoleic acid and protection against tert-butyl peroxyl radical.
Main Results:
- Seven antioxidative compounds were successfully isolated.
- Hydroxyeucomic acid exhibited strong DPPH free radical scavenging activity, comparable to epigallocatechin gallate.
- Orientin and isoorientin displayed significant anti-peroxidative and radical protective effects, similar to epigallocatechin gallate.
Conclusions:
- C. sessiliflora is a valuable source of potent antioxidants.
- Hydroxyeucomic acid, orientin, and isoorientin are key bioactive compounds with significant antioxidant potential.
- These findings support the traditional use and potential therapeutic applications of C. sessiliflora.