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Electrochemical detection of protein-protein interactions using a yeast two hybrid: 17-beta-estradiol as a model

A Schwartz-Mittelmann1, T Neufeld, D Biran

  • 1Department of Molecular Microbiology and Biotechnology, Tel-Aviv University, Ramat-Aviv 69978, Israel.

Analytical Biochemistry
|May 6, 2003
PubMed

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Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...