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Updated: Feb 9, 2026

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
Published on: September 6, 2024
Combined single-molecule force and fluorescence measurements for biology
Mark I Wallace1, Justin E Molloy, David R Trentham
1National Institute for Medical Research, London NW7 1AA, UK.
Abstract:
Recent advances in single-molecule techniques allow the application of force to an individual biomolecule whilst simultaneously monitoring its response using fluorescent probes. The effects of applied mechanical load on single-enzyme turnovers, biomolecular interactions and conformational changes can now be studied with nanometer precision and millisecond time resolution.
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