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Related Concept Videos

Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...

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Related Experiment Video

Updated: Jun 5, 2026

Liquid-cell Transmission Electron Microscopy for Tracking Self-assembly of Nanoparticles
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Real-time label-free imaging of living crystallization-driven self-assembly.

Yujie Guo1, Tianlai Xia2, Vivien Walter3

  • 1Department of Chemistry, King's College London, London, UK.

Nature Communications
|March 19, 2025
PubMed
Summary

Interferometric scattering microscopy visualizes polymer self-assembly in real-time. This technique precisely controls polymer microstructure formation, enabling the engineering of complex hierarchical particles.

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Area of Science:

  • Polymer science and materials engineering
  • Nanotechnology and self-assembly

Background:

  • Living crystallization-driven self-assembly (CDSA) constructs complex polymer microstructures.
  • Precise control over CDSA is crucial for engineering these structures.

Purpose of the Study:

  • To investigate the real-time growth dynamics of polymer structures during CDSA.
  • To understand how reaction parameters influence the size, rate, and morphology of self-assembled polymer particles.
  • To explore the composition distribution in complex, multi-annulus polymer platelets.

Main Methods:

  • Application of interferometric scattering (iSCAT) microscopy for label-free, real-time observation.
  • Monitoring the growth of individual poly(ε-caprolactone)-based fibers and platelets.
  • Utilizing iSCAT's contrast mechanism to study multi-annulus structures.

Main Results:

  • Real-time growth kinetics of polymer fibers and platelets were mapped.
  • Key reaction parameters affecting platelet growth rate, size, and morphology were identified.
  • Insights into the spatial distribution of polymer compositions within multi-annulus platelets were gained.

Conclusions:

  • iSCAT microscopy is a powerful tool for understanding and controlling polymer self-assembly.
  • This method advances the bottom-up construction of uniform polymer microstructures with complex hierarchies.
  • The findings facilitate the precise engineering of advanced polymer materials.