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Updated: May 29, 2026
Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Scanning electron microscopy and atomic force microscopy imaging of solid lipid nanoparticles derived from
Alix Dubes1, Hélène Parrot-Lopez, Wassim Abdelwahed
1Synthèse, Reconnaissance, Organisation Moléculaire et Biomoléculaire (SROMB), CNRS UMR 5078 Université Claude Bernard Lyon1, Cedex, Villeurbanne, France.
Abstract:
Scanning electron microscopy (SEM) and atomic force microscopy (AFM) have been applied to the imagery of solid lipid nanoparticles (SLNs) formulated from an amphiphilic cyclodextrin, 2,3-di-o-alkanoyl-beta-cyclodextrin, beta-CD21C6. Comparison of the results shows that the vacuum drying technique used in sample preparation for SEM causes shrinkage in the size of the SLNs, whereas the deposition method used for AFM causes the SLNs to form small clusters. The hydrodynamic diameter determined from photon correlation spectroscopy (PCS) is 359+/-15 nm and the zeta potential is -25 mV.
