Related Experiment Video
Updated: Aug 8, 2026
13:04
A Protocol for Analyzing Hepatitis C Virus Replication
Published on: June 26, 2014
[Detection of hepatitis C core antigen C33]
1Department of Microbiology of Central Medical Clinical Laboratory of PLA, General Hospital of Nanjing Command of PLA, Nanjing 210002.
Summary
The C33 antigen of Hepatitis C Virus (HCV) was detected in patients with HCV infection and other conditions. Its presence in serum and liver tissues may serve as a marker for HCV infection.
Area of Science:
- Hepatology
- Immunology
- Virology
Context:
- Hepatitis C Virus (HCV) infection remains a significant global health concern.
- Accurate diagnostic markers are crucial for timely detection and management of HCV.
- Understanding viral antigen distribution aids in comprehending disease pathogenesis.
Purpose:
- To investigate the distribution of the C33 antigen of HCV in serum and liver tissues.
- To evaluate the C33 antigen as a potential marker for HCV infection.
Summary:
- Monoclonal antibodies were developed against the HCV core antigen peptide C33.
- An ELISA detected C33 antigen in 14.0% of acute hepatitis C patients and 8.8% of anti-HCV-IgG positive individuals.
- Immunohistochemistry and immunofluorescence revealed C33 antigen in hepatic cells of patients.
Impact:
- The study identifies the C33 antigen as a potential diagnostic marker for HCV infection.
- Findings contribute to the understanding of HCV antigen localization in infected tissues.
- This research may inform the development of novel diagnostic strategies for Hepatitis C.
